GSM1704601: 72 h DENV1 infection Total cell lysate mRNA-seq (rep 1); ... - SRA

SRX1050426: GSM1704601: 72 h DENV1 infection Total cell lysate mRNA-seq (rep 1); Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 46.6M spots, 2.4G bases, 1.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Ribosome profiling analysis of Dengue Virus

PRJNA285961 • SRP059187 • All experiments • All runs

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Dengue virus is an + strand RNA virus. We have carried our infections of human cells with Dengue and analyzed the translation, replication, and localization of the Dengue RNA. This allowed for clear definition of the life cycle of the Dengue virus inside a host cell. We also assessed the host response to Dengue virus, finding that a large fraction of the translational response is due to Interferon function. Overall design: Translational and transcriptional analysis of the cellular response to Dengue virus infection

Sample: 72 h DENV1 infection Total cell lysate mRNA-seq (rep 1)

SAMN03763437 • SRS954556 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. For fractionated cells, release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. NEB Directional Small RNA kit PNK end polishing, linker ligation, reverse transcription, PCR amplification

Experiment attributes:

GEO Accession: GSM1704601

Links:

External link: GEO Sample GSM1704601

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 46.6M spots, 2.4G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR2053009	46,558,286	2.4G	1.5Gb	2016-09-01

ID:: 1525574

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